Reference - Detail
| RRC ID | 45317 |
|---|---|
| Author | Munisso MC, Obika S, Yamaoka T. |
| Title | Nucleic acid delivery systems based on poly(galactosyl ureaethyl methacrylate-b-dimethylamino ethyl methacrylate) diblock copolymers. |
| Journal | Carbohydr Polym |
| Abstract |
Elevated low-density-lipoprotein cholesterol (LDL-C) level is a major risk factor leading to cardiovascular diseases. Therefore, since the proprotein convertase subtilisin kexin type 9 (PCSK9) regulates LDL-C receptors, it represents the appropriate target for cholesterol-lowering gene therapy. However, although delivery of antisense oligonucleotides (ODNs) is a promising therapeutic method for the treatment of several diseases, it is fundamental to develop new and efficacious carriers that transport the ODNs into the target cell in a nontoxic manner. This study reports on the synthesis, characterization and in vitro testing of a new liver specific carrier based on linear cationic diblock glycopolymer composed of galactosyl ureaethyl methacrylate (GAMA) and the primary amine-containing dimethylamino ethyl methacrylate (DMAEMA). Delivery experiments proved that the poly(galactosyl ureaethyl methacrylate -b-dimethylamino ethyl methacrylate) diblock copolymer (pGa4D47), internalized in a receptor-mediated manner, exhibited a much faster nuclear transportation than ODNs carried by pDMAEMA homopolymer or glycopolymer bearing glucose moieties. |
| Volume | 114 |
| Pages | 288-296 |
| Published | 2014-12-19 |
| DOI | 10.1016/j.carbpol.2014.08.022 |
| PII | S0144-8617(14)00791-7 |
| PMID | 25263893 |
| MeSH | Cell Survival Gene Transfer Techniques* Genetic Therapy HeLa Cells Hep G2 Cells Humans Magnetic Resonance Spectroscopy Methacrylates / chemistry Nucleic Acids / administration & dosage* Nucleic Acids / chemistry* Nylons / chemistry Polymers / chemistry* |
| IF | 7.182 |
| Times Cited | 3 |
| WOS Category | POLYMER SCIENCE CHEMISTRY, APPLIED CHEMISTRY, ORGANIC |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Human and Animal Cells | Hep G2 |