RRC ID 45337
Author Ivashchenko S, Escobar Ivirico JL, García Cruz DM, Campillo-Fernández A, Gallego Ferrer G, Monleón Pradas M.
Title Bioactive organic-inorganic poly(CLMA-co-HEA)/silica nanocomposites.
Journal J Biomater Appl
Abstract A series of novel poly(CLMA-co-HEA)/silica nanocomposites is synthesized from caprolactone 2-(methacryloyloxy)ethyl ester (CLMA) and 2-hydroxyethyl acrylate (HEA) as organic comonomers and the simultaneous sol-gel polymerization of tetraethyloxysilane (TEOS) as silica precursor, in different mass ratios up to a 30 wt% of silica. The nanocomposites are characterized as to their mechanical and thermal properties, water sorption, bioactivity and biocompatibility, reflecting the effect on the organic matrix provided by the silica network formation. The nanocomposites nucleate the growth of hydroxyapatite (HAp) on their surfaces when immersed in the simulated body fluid of the composition used in this work. Proliferation of the MC3T3 osteoblast-like cells on the materials was assessed with the MTS assay showing their biocompatibility. Immunocytochemistry reveals osteocalcin and type I collagen production, indicating that osteoblast differentiation was promoted by the materials, and calcium deposition was confirmed by von Kossa staining. The results indicate that these poly(CLMA-co-HEA)/silica nanocomposites could be a promising biomaterial for bone tissue engineering.
Volume 29(8)
Pages 1096-108
Published 2015-3-1
DOI 10.1177/0885328214554816
PII 0885328214554816
PMID 25294191
MeSH 3T3 Cells Animals Biocompatible Materials / chemistry* Calcium / metabolism Cell Differentiation Cell Proliferation Collagen Type I / metabolism Materials Testing Mice Microscopy, Electron, Scanning Nanocomposites / chemistry* Nanocomposites / ultrastructure Osteoblasts / cytology Osteoblasts / metabolism Osteocalcin / metabolism Polyesters / chemistry* Polyethylene Glycols / chemistry Silicon Dioxide / chemistry* Tissue Engineering / methods
IF 2.442
Times Cited 2
Human and Animal Cells