Reference - Detail
| RRC ID | 45348 |
|---|---|
| Author | Nakayama T, Saitoh H. |
| Title | Tunicamycin-induced neutrophil extracellular trap (NET)-like structures in cultured human myeloid cell lines. |
| Journal | Cell Biol Int |
| Abstract |
The mechanism of neutrophil extracellular trap cell death (NETosis), a regulated cell death pathway relevant to infection, autoimmunity and sepsis, is not completely known. The reason for this, at least in part, is the lack of an in vitro system that recapitulates the NETosis pathway using established human cell lines. We show that exposure of a human promyelocytic leukemia cell line HL-60 to the glycosyltransferase inhibitor tunicamycin (TM) resulted in extrusion of decompacted genomic DNAs to extracellular space, morphologically similar to NETs. Immunostaining using antibodies against NET marker proteins and bacterial trapping assay showed biochemical similarities between the TM-induced extracellular DNA structures and NETs. The NET-like structures were also generated on exposure of TM to other myeloid cell lines, such as U937 and THP-1. Thus, our findings provide an experimental setting to induce NET-like structures using cultured human myeloid cell lines, which may help our understanding of the regulation and function of NETosis. |
| Volume | 39(3) |
| Pages | 355-9 |
| Published | 2015-3-1 |
| DOI | 10.1002/cbin.10396 |
| PMID | 25336229 |
| MeSH | Anti-Bacterial Agents / pharmacology* Apoptosis / drug effects Cell Line, Tumor Extracellular Traps / chemistry Extracellular Traps / drug effects* Extracellular Traps / metabolism Glycosyltransferases / antagonists & inhibitors Glycosyltransferases / metabolism HL-60 Cells Humans Neutrophils / pathology Tunicamycin / pharmacology* |
| IF | 2.571 |
| Times Cited | 7 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa 293(RCB1637) |