| RRC ID |
45353
|
| 著者 |
Tano K, Yasuda S, Kuroda T, Saito H, Umezawa A, Sato Y.
|
| タイトル |
A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.
|
| ジャーナル |
PLoS One
|
| Abstract |
Innovative applications of cell therapy products (CTPs) derived from human pluripotent stem cells (hPSCs) in regenerative medicine are currently being developed. The presence of residual undifferentiated hPSCs in CTPs is a quality concern associated with tumorigencity. However, no simple in vitro method for direct detection of undifferentiated hPSCs that contaminate CTPs has been developed. Here, we show a novel approach for direct and sensitive detection of a trace amount of undifferentiated human induced pluripotent stem cells (hiPSCs) using a highly efficient amplification method in combination with laminin-521 and Essential 8 medium. Essential 8 medium better facilitated the growth of hiPSCs dissociated into single cells on laminin-521 than in mTeSR1 medium. hiPSCs cultured on laminin-521 in Essential 8 medium were maintained in an undifferentiated state and they maintained the ability to differentiate into various cell types. Essential 8 medium allowed robust hiPSC proliferation plated on laminin-521 at low cell density, whereas mTeSR1 did not enhance the cell growth. The highly efficient culture system using laminin-521 and Essential 8 medium detected hiPSCs spiked into primary human mesenchymal stem cells (hMSCs) or human neurons at the ratio of 0.001%-0.01% as formed colonies. Moreover, this assay method was demonstrated to detect residual undifferentiated hiPSCs in cell preparations during the process of hMSC differentiation from hiPSCs. These results indicate that our highly efficient amplification system using a combination of laminin-521 and Essential 8 medium is able to detect a trace amount of undifferentiated hPSCs contained as impurities in CTPs and would contribute to quality assessment of hPSC-derived CTPs during the manufacturing process.
|
| 巻・号 |
9(10)
|
| ページ |
e110496
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0110496
|
| PII |
PONE-D-14-14050
|
| PMID |
25347300
|
| PMC |
PMC4210199
|
| MeSH |
Animals
Cell Culture Techniques*
Cell Differentiation*
Cell Line
Cell Proliferation
Cell- and Tissue-Based Therapy*
Culture Media / chemistry
Humans
In Vitro Techniques*
Induced Pluripotent Stem Cells / cytology
Laminin
Mice
Pluripotent Stem Cells / cytology*
|
| IF |
2.74
|
| 引用数 |
24
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063)
253G1(HPS0002)
409B2(HPS0076) |
