RRC ID 45362
Author Ribeiro C, Sencadas V, Areias AC, Gama FM, Lanceros-Méndez S.
Title Surface roughness dependent osteoblast and fibroblast response on poly(L-lactide) films and electrospun membranes.
Journal J Biomed Mater Res A
Abstract Poly(l-lactide) electrospun mats with random and aligned fiber orientation and films have been produced with degrees of crystallinity ranging from 0 up to nearly 50%. The overall surface roughness is practically constant irrespective of the sampling areas (1 × 1 µm to 20 × 20 µm) for degrees of crystallinity below 30%, increasing for higher degrees of crystallinity for the larger sampling areas. Further, due to fiber confinement, surface roughness variations are smaller in electrospun mats. Samples with 50% of crystallinity show the lowest osteoblast and the highest fibroblast proliferation. Therefore, it is verified that higher roughness promotes lower osteoblast but higher fibroblast proliferation. The overall results indicate the relevant role of the sub-microenvironment variations associated to the microscale roughness in determining the different cell responses.
Volume 103(7)
Pages 2260-8
Published 2015-7-1
DOI 10.1002/jbm.a.35367
PMID 25370449
MeSH 3T3 Cells Animals Cell Proliferation Crystallization Fibroblasts / cytology* Mice Osteoblasts / cytology* Polyesters / chemistry* Surface Properties
IF 3.221
Times Cited 30
Human and Animal Cells