| RRC ID |
45375
|
| 著者 |
George KA, Shadforth AM, Chirila TV, Laurent MJ, Stephenson SA, Edwards GA, Madden PW, Hutmacher DW, Harkin DG.
|
| タイトル |
Effect of the sterilization method on the properties of Bombyx mori silk fibroin films.
|
| ジャーナル |
Mater Sci Eng C Mater Biol Appl
|
| Abstract |
We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.
|
| 巻・号 |
33(2)
|
| ページ |
668-74
|
| 公開日 |
2013-3-1
|
| DOI |
10.1016/j.msec.2012.10.016
|
| PII |
S0928-4931(12)00479-1
|
| PMID |
25427472
|
| MeSH |
Animals
Biocompatible Materials / chemistry*
Biocompatible Materials / pharmacology
Bombyx*
Cell Adhesion / drug effects
Cell Culture Techniques / methods*
Cell Line
Fibroins / chemistry*
Fibroins / pharmacology
Fibroins / physiology
Humans
Sterilization
Surface Properties
Tensile Strength / physiology
Tissue Engineering
|
| IF |
5.88
|
| 引用数 |
17
|
|
WOS 分野
|
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
HCE-T(RCB2280) |
