論文 - 詳細
| RRC ID | 45377 |
|---|---|
| 著者 | Park E, Jin HS, Cho DY, Kim J, Kim MC, Choi CW, Jin Y, Lee JW, Park JH, Chung YS, Huh D, Jeong SY. |
| タイトル | The effect of Lycii Radicis Cortex extract on bone formation in vitro and in vivo. |
| ジャーナル | Molecules |
| Abstract |
Osteoporosis is a common skeletal disease caused by decreased bone mass; it enhances the risk of bone fracture. This study aimed to discover novel herbal extract(s) for the treatment of osteoporosis. We screened 64 ethanol extracts of edible plants native to Korea for their ability to increase the cellular proliferation and differentiation of two osteoblastic cell lines: C3H10T1/2 and MC3T3-E1. We selected a Lycii Radicis Cortex (LRC), Lycium Chinese root bark as the primary candidate. Treatment with LRC extract showed enhanced alkaline phosphatase activity and increased expression of bone metabolic markers Alpl, Runx2, and Bglap genes in both osteoblastic cell lines. There was no effect on the osteoclastic differentiation of primary-cultured monocytes from the mouse bone marrows. Furthermore, the study examined the effect of LRC extract in vivo in ovariectomizd (OVX) mice for 8 weeks and 16 weeks, respectively. Bone mineral density (BMD) was significantly higher in LRC extract-administered group than in the non-LRC-administered OVX control group. The results indicated that LRC extract prevented the OVX-induced BMD loss in mice via promoting the differentiation of osteoblast linage cells. These results suggest that LRC extract may be a good natural herbal medicine candidate for the treatment of osteoporosis. |
| 巻・号 | 19(12) |
| ページ | 19594-609 |
| 公開日 | 2014-11-26 |
| DOI | 10.3390/molecules191219594 |
| PII | molecules191219594 |
| PMID | 25432011 |
| PMC | PMC6271141 |
| MeSH | Animals Biomarkers / metabolism Bone Density / drug effects Cell Differentiation / drug effects Cell Line Cell Proliferation / drug effects Chromatography, High Pressure Liquid Drugs, Chinese Herbal / chemistry Drugs, Chinese Herbal / pharmacology* Female Lycium / chemistry Mice Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism Osteogenesis / drug effects* Ovariectomy Plant Extracts / chemistry Plant Extracts / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Republic of Korea Spectrometry, Mass, Electrospray Ionization |
| IF | 3.267 |
| 引用数 | 7 |
| WOS 分野 | CHEMISTRY, ORGANIC |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |