RRC ID 45398
Author Horie M, Ito A, Maki T, Kawabe Y, Kamihira M.
Title Magnetically labeled feeder system for mouse pluripotent stem cell culture.
Journal J Biosci Bioeng
Abstract We report here a magnetically labeled feeder system for mouse embryonic stem/induced pluripotent stem (ES/iPS) cells. Magnetic attraction of feeder cells labeled with magnetite nanoparticles significantly increased ES/iPS colony-forming efficiency. Magnetic labeling of feeder cells also facilitated separation of ES/iPS cells from feeder cells.
Volume 119(5)
Pages 614-6
Published 2015-5-1
DOI 10.1016/j.jbiosc.2014.10.020
PII S1389-1723(14)00398-3
PMID 25468421
MeSH Animals Cadherins / genetics Cell Culture Techniques / methods* Cell Separation / methods* Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Feeder Cells / cytology* Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Magnetite Nanoparticles / analysis* Magnetite Nanoparticles / chemistry Mice Staining and Labeling / methods*
IF 2.366
Times Cited 2
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY FOOD SCIENCE & TECHNOLOGY
Resource
Human and Animal Cells iPS-MEF-Ng-20D-17(APS0001)