RRC ID 45408
Author Roeen Z, Toda M, D'Alessandro-Gabazza CN, Onishi M, Kobayashi T, Yasuma T, Urawa M, Taguchi O, Gabazza EC.
Title Thrombomodulin inhibits the activation of eosinophils and mast cells.
Journal Cell. Immunol.
Abstract Eosinophils and mast cells play critical roles in the pathogenesis of bronchial asthma. Activation of both cells leads to the release of pro-inflammatory mediators in the airway of asthmatic patients. Recently, we have shown that inhaled thrombomodulin inhibits allergic bronchial asthma in a mouse model. In the present study, we hypothesize that thrombomodulin can inhibit the activation of eosinophils and mast cells. The effect of thrombomodulin on the activation and release of inflammatory mediators from eosinophils and mast cells was evaluated. Thrombomodulin inhibited the eotaxin-induced chemotaxis, upregulation of CD11b and degranulation of eosinophils. Treatment with thrombomodulin also significantly suppressed the degranulation and synthesis of inflammatory cytokines and chemokines in eosinophils and mast cells. Mice treated with a low-dose of inhaled thrombomodulin have decreased number of eosinophils and activated mast cells and Th2 cytokines in the lungs compared to untreated mice. The results of this study suggest that thrombomodulin may modulate allergic responses by inhibiting the activation of both eosinophils and mast cells.
Volume 293(1)
Pages 34-40
Published 2015-1
DOI 10.1016/j.cellimm.2014.11.005
PII S0008-8749(14)00175-0
PMID 25497974
MeSH Administration, Inhalation Animals Asthma / chemically induced Asthma / drug therapy* Asthma / immunology Asthma / pathology CD11b Antigen / genetics CD11b Antigen / immunology Cell Degranulation Cell Line, Tumor Chemokine CCL11 / drug effects Chemokine CCL11 / metabolism Chemotaxis / drug effects Cytokines / biosynthesis Cytokines / metabolism Eosinophils / drug effects* Eosinophils / immunology Eosinophils / pathology Gene Expression Humans Mast Cells / drug effects* Mast Cells / immunology Mast Cells / pathology Mice Ovalbumin Primary Cell Culture Recombinant Proteins / administration & dosage Th1-Th2 Balance Thrombomodulin / administration & dosage*
IF 2.995
Times Cited 2
Human and Animal Cells