RRC ID 45414
Author Subramanian N, Kanwar JR, Kanwar RK, Krishnakumar S.
Title Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma.
Journal Nucleic Acid Ther
Abstract The miR-17∼92. or oncomiR-1, cluster encodes oncogenic microRNAs (miRNAs), and it also promotes retinoblastoma (RB) tumor formation. Antagomir and miRNA mimics based approaches are widely tried against oncogenic and tumor suppressive miRNAs. Other methods for targeting cancer related miRNAs are still under development. In the current study, we focused on the pri-miRNA-17∼92 aptamer (pri-apt), which can potentially replace the mix of five antagomirs by one aptamer that function to abrogate the maturation of miR-17, miR-18a, and miR-19b (P<0.05) for targeting RB. We used RB cell lines WERI-Rb1 and Y79 as an in vitro model. Cellular changes upon transfecting the pri-apt led to S-phase arrest in WERI-Rb1 cells and onset of apoptosis in both Y79 and WERI-Rb1 cell lines. There was increased cytotoxicity as measured by lactate dehydrogenase activity in pri-apt treated Y79 cells (P<0.05), and significant inhibition of cell proliferation was observed in both of the cell lines. Thus we showed the antiproliferative property of pri-apt in RB cell lines, which can be readily modified by developing appropriate vectors for the delivery of the aptamer specifically to cancer cells.
Volume 25(1)
Pages 47-52
Published 2015-2-1
DOI 10.1089/nat.2014.0507
PMID 25513843
PMC PMC4296747
MeSH Apoptosis Aptamers, Nucleotide / genetics* Cell Line, Tumor Cell Proliferation Gene Expression Regulation, Neoplastic Genetic Therapy Humans MicroRNAs / genetics* RNA Interference RNA, Long Noncoding Retinoblastoma / genetics Retinoblastoma / therapy
IF 5.0
Times Cited 8
Human and Animal Cells Y79(RCB1645) WERI-Rb-1(RCB2146)