| RRC ID |
45423
|
| Author |
Manaka S, Tanabe N, Kariya T, Naito M, Takayama T, Nagao M, Liu D, Ito K, Maeno M, Suzuki N, Miyazaki M.
|
| Title |
Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells.
|
| Journal |
FEBS Lett
|
| Abstract |
Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.
|
| Volume |
589(3)
|
| Pages |
310-8
|
| Published |
2015-1-30
|
| DOI |
10.1016/j.febslet.2014.12.013
|
| PII |
S0014-5793(14)00893-X
|
| PMID |
25542352
|
| MeSH |
Adenosine Triphosphate / genetics
Adenosine Triphosphate / metabolism
Alkaline Phosphatase / metabolism
Animals
Cell Differentiation / genetics*
Cell Differentiation / radiation effects
Cell Proliferation / radiation effects
Humans
Mice
Osteoblasts / metabolism
Osteoblasts / radiation effects
Osteogenesis / genetics*
Osteogenesis / radiation effects
Receptors, Purinergic P2X7 / genetics
Receptors, Purinergic P2X7 / metabolism*
Sound*
|
| IF |
3.057
|
| Times Cited |
24
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
MC3T3-E1(RCB1126) |
