論文 - 詳細
RRC ID | 45451 |
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著者 | Hashimoto Y, Nishikawa H, Kusunoki M, Li P, Hontsu S. |
タイトル | A novel membrane-type apatite scaffold engineered by pulsed laser ablation. |
ジャーナル | Dent Mater J |
Abstract |
Cell sheet technology is a scaffold-free method for tissue reconstruction. A sheet-shaped scaffold would be suitable for the regeneration of periodontal membrane. We designed a stem cell sheet combining human mesenchymal stromal cells (hMSCs) and a 10-µm thick biological apatite (BAp) membrane fabricated with an ArF pulsed laser ablation for periodontal regeneration. X-ray diffraction showed that crystalline hydroxyapatite (HAp) was present in BAp and HAp membranes after post-annealing. Energy dispersive analysis of the BAp membrane revealed peaks of Na and Mg in addition to Ca and P. Approximately 3×10(4) hMSCs were cultured on BAp and HAP membranes for 7 and 14 days. From in vitro assays, hMSCs grew faster and had higher osteoblast differentiation when cultured on the BAp membrane than did the cell culture on the HAp membrane. Stem cell sheets combined with a BAp membrane may have potential applications in guided bone regeneration and osteoconductive scaffolds. |
巻・号 | 34(3) |
ページ | 345-50 |
公開日 | 2015-1-1 |
DOI | 10.4012/dmj.2014-299 |
PMID | 25904175 |
MeSH | Ablation Techniques* Apatites / chemistry* Cell Differentiation Equipment Design Humans Lasers* Materials Testing Mesenchymal Stem Cells / cytology* Osteoblasts / cytology* Spectrometry, X-Ray Emission Tissue Engineering / methods* Tissue Scaffolds / chemistry* |
IF | 1.359 |
引用数 | 3 |
WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS |
リソース情報 | |
ヒト・動物細胞 | Human mesenchymal stem cells |