論文 - 詳細
| RRC ID | 45459 |
|---|---|
| 著者 | Kato S, Kimura M, Miwa N. |
| タイトル | Enhanced radiosensitization by the cationic liposome-encapsulated thymidine analogue BrdU through the increased intracellular BrdU-uptake on human melanoma as compared to anionic or nonionic liposomal or free BrdU. |
| ジャーナル | J Biomed Nanotechnol |
| Abstract |
The synthetic thymidine analogue, 5-bromo-2'-deoxy-uridine (BrdU) was encapsulated in cationic liposome composed of dipalmitoylphosphatidylcholine, cholesterol and stearylamine (molar ratio = 1/1/0.2; diameter = 120 nm), and the radiosensitization of cationic liposomal BrdU was assessed on human melanoma cells HMV-II, with comparing to anionic or nonionic liposomal BrdU and free-BrdU. HMV-II cells were pretreated by cationic liposomal BrdU or free-BrdU and then exposed to X-ray, followed by WST-8 assay to examine cell proliferation. The radiation-induced change of nuclei was defined with Hoechst33342 staining. The rates of thymidine replacement by BrdU and DNA double-strand breaks on HMV-II cells were determined with an anti-BrdU antibody and anti-53BP1 antibody, respectively. On 7th day after X-ray irradiation at 3 Gy, cell proliferation was suppressed more markedly in the administration of cationic liposomal BrdU than free-BrdU at equivalent BrdU doses. Giant polykaryocytes were observed in cationic liposomal BrdU-treated HMV-II cells. Radiosensitization was enhanced dose-dependently along with BrdU doses of 0.1-0.8 μM in the order: cationic liposomal BrdU > anionic liposomal BrdU > nonionic liposomal BrdU (see symbol) free-BrdU. Similarly, the cationic liposomal BrdU augmented the rate of thymidine-moiety replacement by BrdU and DNA double-strand breaks more appreciably than free-BrdU. Therefore, the cationic liposome-encapsulation of BrdU would be one of favorable drug deliveries for facilitating the X-ray therapy against cancer. |
| 巻・号 | 10(11) |
| ページ | 3280-90 |
| 公開日 | 2014-11-1 |
| DOI | 10.1166/jbn.2014.1869 |
| PMID | 26000387 |
| MeSH | Antimetabolites, Antineoplastic / administration & dosage Antimetabolites, Antineoplastic / chemistry Bromodeoxyuridine / administration & dosage* Bromodeoxyuridine / chemistry Bromodeoxyuridine / pharmacokinetics* Cations Cell Line, Tumor Diffusion Humans Liposomes / chemistry* Melanoma / drug therapy* Melanoma / metabolism* Melanoma / pathology Metabolic Clearance Rate Nanocapsules / chemistry* Nanocapsules / ultrastructure Radiation Tolerance Radiation-Sensitizing Agents / administration & dosage* Radiation-Sensitizing Agents / chemistry Treatment Outcome |
| IF | 4.483 |
| 引用数 | 3 |
| WOS 分野 | NANOSCIENCE & NANOTECHNOLOGY MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HMV-II(RCB0777) |