Reference - Detail
| RRC ID | 45474 |
|---|---|
| Author | Tahara Y, Yasuoka J, Sawada S, Sasaki Y, Akiyoshi K. |
| Title | Effective CpG DNA delivery using amphiphilic cycloamylose nanogels. |
| Journal | Biomater Sci |
| Abstract |
Unmethylated CpG oligodeoxynucleotides induce inflammatory immune responses through cytokine production and have attracted increasing attention as an immunostimulator. However, there remains a challenging issue of the use of 'native CpG DNA'. In the present study, we prepared cationic nanometer-sized gels (nanogels) consisting of cycloamylose modified with cholesterol and diethylaminoethane to form hydrophobic cross-linking points and to add positively charged groups, respectively. The cationic nanogels and native CpG DNA formed nanometer-sized complexes. Complexes of native CpG DNA with cationic nanogels delivered native CpG DNA to macrophage-like cells and induced cytokine production. In addition, complexes of negative control oligonucleotides with cationic nanogels did not induce cytokine production, and the induction of cytokines using complexes of phosphorothioate-modified CpG with cationic nanogels was lower than that of native CpG DNA. These results suggest that the complex of native CpG DNA with cationic nanogels is a promising strategy for nucleic acid adjuvants. |
| Volume | 3(2) |
| Pages | 256-64 |
| Published | 2015-2-1 |
| DOI | 10.1039/c4bm00293h |
| PMID | 26218116 |
| MeSH | Cations Cholesterol / chemistry* Cyclodextrins / chemistry* Cyclodextrins / metabolism Cytokines / chemistry* Cytokines / metabolism DNA / administration & dosage DNA / chemistry* Drug Carriers Ethylamines / chemistry Liposomes / chemistry* Liposomes / metabolism Macrophages / chemistry* Macrophages / drug effects Macrophages / metabolism* Nanogels Oligodeoxyribonucleotides Phosphorothioate Oligonucleotides / chemistry* Phosphorothioate Oligonucleotides / metabolism Polyethylene Glycols / chemistry* Polyethyleneimine / chemistry* |
| IF | 6.183 |
| Times Cited | 10 |
| WOS Category | MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |