RRC ID 45487
Author Tamura M, Yanagawa F, Sugiura S, Takagi T, Sumaru K, Kanamori T.
Title Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment.
Journal Sci Rep
Abstract This paper describes the generation of "click-crosslinkable" and "photodegaradable" gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were created by micropatterned light irradiation; the minimum resolution of micropatterning was 10-μm widths for line patterns and 20-μm diameters for circle patterns. Cells were successfully encapsulated in the hydrogels without any loss of viability across a wide concentration range of crosslinker. In contrast, an activated-ester-type photocleavable crosslinker, which we previously used to prepare photodegradable gelatin hydrogels, induced a decrease in cell viability at crosslinker concentrations greater than 1.8 mM. We also observed morphology alteration and better growth of cancer cells in the click-crosslinked photodegradable gelatin hydrogels that included matrigel than in the absence of matrigel. We also demonstrated micropatterning of the hydrogels encapsulating cells and optical cell separation. Both of the cells that remained in the non-irradiated area and the cells collected from the irradiated area maintained their viability.
Volume 5
Pages 15060
Published 2015-10-9
DOI 10.1038/srep15060
PII srep15060
PMID 26450015
PMC PMC4598855
MeSH Azides / chemistry Cell Line, Tumor Cell Survival / drug effects Click Chemistry / methods* Collagen Cross-Linking Reagents / chemistry* Drug Combinations Gelatin / chemistry* HeLa Cells Humans Hydrogels / chemistry* Hydrogels / pharmacology Laminin Light Models, Chemical Molecular Structure Photolysis / radiation effects Polyethylene Glycols / chemistry Proteoglycans Succinimides / chemistry
IF 3.998
Times Cited 35
Human and Animal Cells DU145(RCB2143) HeLa(RCB0007)