| RRC ID |
45487
|
| 著者 |
Tamura M, Yanagawa F, Sugiura S, Takagi T, Sumaru K, Kanamori T.
|
| タイトル |
Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment.
|
| ジャーナル |
Sci Rep
|
| Abstract |
This paper describes the generation of "click-crosslinkable" and "photodegaradable" gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydrogels were formed in 30 min through the click-crosslinking reaction. The micropatterned features in the hydrogels were created by micropatterned light irradiation; the minimum resolution of micropatterning was 10-μm widths for line patterns and 20-μm diameters for circle patterns. Cells were successfully encapsulated in the hydrogels without any loss of viability across a wide concentration range of crosslinker. In contrast, an activated-ester-type photocleavable crosslinker, which we previously used to prepare photodegradable gelatin hydrogels, induced a decrease in cell viability at crosslinker concentrations greater than 1.8 mM. We also observed morphology alteration and better growth of cancer cells in the click-crosslinked photodegradable gelatin hydrogels that included matrigel than in the absence of matrigel. We also demonstrated micropatterning of the hydrogels encapsulating cells and optical cell separation. Both of the cells that remained in the non-irradiated area and the cells collected from the irradiated area maintained their viability.
|
| 巻・号 |
5
|
| ページ |
15060
|
| 公開日 |
2015-10-9
|
| DOI |
10.1038/srep15060
|
| PII |
srep15060
|
| PMID |
26450015
|
| PMC |
PMC4598855
|
| MeSH |
Azides / chemistry
Cell Line, Tumor
Cell Survival / drug effects
Click Chemistry / methods*
Collagen
Cross-Linking Reagents / chemistry*
Drug Combinations
Gelatin / chemistry*
HeLa Cells
Humans
Hydrogels / chemistry*
Hydrogels / pharmacology
Laminin
Light
Models, Chemical
Molecular Structure
Photolysis / radiation effects
Polyethylene Glycols / chemistry
Proteoglycans
Succinimides / chemistry
|
| IF |
3.998
|
| 引用数 |
35
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
DU145(RCB2143)
HeLa(RCB0007) |
