Reference - Detail
| RRC ID | 45503 |
|---|---|
| Author | Taniguchi T, Yamazaki W, Saeki Y, Takajo I, Okayama A, Hayashi T, Misawa N. |
| Title | The pathogenic potential of Helicobacter cinaedi isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells in vitro. |
| Journal | J Vet Med Sci |
| Abstract |
Helicobacter cinaedi infection has been recognized as an increasingly important emerging disease in humans. Infection with H. cinaedi causes bacteremia, cellulitis and enteritis. H. cinaedi has been isolated from non-human sources, including dogs, cats and rodents; however, it remains unclear whether animal strains are pathogenic in humans and as zoonotic pathogens. In this study, H. cinaedi isolates were recovered from a dog and a hamster, and the ability of these isolates to adhere to, invade and translocate across polarized human intestinal epithelial Caco-2 cells was examined in vitro. To better understand the pathogenic potential of animal H. cinaedi isolates, these results were compared with those for a human strain that was isolated from a patient with bacteremia. The animal and human strains adhered to and invaded Caco-2 cells, but to a lesser degree than the C. jejuni 81-176 strain, which was used as a control. The integrity of tight junctions was monitored by measuring transepithelial electrical resistance (TER) with a membrane insert system. The TER values for all H. cinaedi strains did not change during the experimental periods compared with those of the controls; however, translocation of H. cinaedi from the apical side to the basolateral side was confirmed by cultivation and H. cinaedi-specific PCR, suggesting that the H. cinaedi strains translocated by transcellular route. This study demonstrated that H. cinaedi strains of animal origin might have a pathogenic potential in human epithelial cells as observed in a translocation assay in vitro with a human isolate. |
| Volume | 78(4) |
| Pages | 627-32 |
| Published | 2016-5-3 |
| DOI | 10.1292/jvms.15-0595 |
| PMID | 26685883 |
| PMC | PMC4873854 |
| MeSH | Animals Bacterial Adhesion Caco-2 Cells Cell Polarity Cricetinae Dogs Helicobacter / isolation & purification Helicobacter / pathogenicity* Humans Intestinal Mucosa / microbiology* |
| IF | 1.049 |
| Times Cited | 5 |
| WOS Category | VETERINARY SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CACO-2(RCB0988) |