論文 - 詳細
| RRC ID | 45541 |
|---|---|
| 著者 | Choudhary V, Ojha N, Golden A, Prinz WA. |
| タイトル | A conserved family of proteins facilitates nascent lipid droplet budding from the ER. |
| ジャーナル | J Cell Biol |
| Abstract |
Lipid droplets (LDs) are found in all cells and play critical roles in lipid metabolism. De novo LD biogenesis occurs in the endoplasmic reticulum (ER) but is not well understood. We imaged early stages of LD biogenesis using electron microscopy and found that nascent LDs form lens-like structures that are in the ER membrane, raising the question of how these nascent LDs bud from the ER as they grow. We found that a conserved family of proteins, fat storage-inducing transmembrane (FIT) proteins, is required for proper budding of LDs from the ER. Elimination or reduction of FIT proteins in yeast and higher eukaryotes causes LDs to remain in the ER membrane. Deletion of the single FIT protein in Caenorhabditis elegans is lethal, suggesting that LD budding is an essential process in this organism. Our findings indicated that FIT proteins are necessary to promote budding of nascent LDs from the ER. |
| 巻・号 | 211(2) |
| ページ | 261-71 |
| 公開日 | 2015-10-26 |
| DOI | 10.1083/jcb.201505067 |
| PII | jcb.201505067 |
| PMID | 26504167 |
| PMC | PMC4621845 |
| MeSH | 3T3 Cells Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Cell Line Endoplasmic Reticulum / metabolism* Genes, Lethal Lipid Droplets / metabolism* Lipid Metabolism / physiology* Membrane Proteins / genetics* Membrane Proteins / metabolism Mice Microscopy, Electron RNA Interference RNA, Small Interfering |
| IF | 8.811 |
| 引用数 | 100 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3632 |