論文 - 詳細
| RRC ID | 456 |
|---|---|
| 著者 | Suzuki Y, Matsuoka T, Iimura Y, Fujiwara H. |
| タイトル | Ecdysteroid-dependent expression of a novel cuticle protein gene BMCPG1 in the silkworm, Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
When insects molt, the exoskeleton is renewed under the controls of insect hormones via the biosynthesis and degradation of cuticle proteins. To understand the hormonal control of cuticle formation, we used the differential display method to look for stage-specific cuticle genes, and identified a novel cDNA named Bombyx mori Cuticle Protein GlyGlyTyr-repeat 1 (BMCPG1). Expression of BMCPG1 mRNA peaked sharply immediately after a pulse of ecdysteroid during the fourth molt and pre-pupal stages, concurrent with the expression of genes for FTZF1 and dopa decarboxylase. BMCPG1 was expressed only in the epidermis, but not in any other tissue. We cultured the larval epidermis and found that BMCPG1 expression is not induced by the continuous presence of ecdysteroid. Removal of ecdysteroid from the medium, which constitutes a pulse treatment, is required for the induction of BMCPG1 transcription. These results explain well the stage-specific expression of BMCPG1 by ecdysteroid in vivo. Based on its expression patterns and unique structure, we propose that BMCPG1 may be a novel component of epicuticle of B. mori, and is probably involved in cross-linking of proteins via its GGY repeats. |
| 巻・号 | 32(6) |
| ページ | 599-607 |
| 公開日 | 2002-6-1 |
| DOI | 10.1016/s0965-1748(01)00136-9 |
| PII | S0965174801001369 |
| PMID | 12020834 |
| MeSH | Amino Acid Sequence Animals Base Sequence Bombyx / drug effects Bombyx / genetics* Bombyx / growth & development DNA, Complementary Ecdysterone / metabolism* Ecdysterone / pharmacology Epidermis / metabolism Gene Expression* Insect Proteins / genetics* Metamorphosis, Biological Molecular Sequence Data Molting Sequence Homology, Amino Acid Tissue Distribution |
| IF | 3.827 |
| 引用数 | 30 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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