論文 - 詳細
| RRC ID | 45611 |
|---|---|
| 著者 | Matsunaga Y, Qadota H, Furukawa M, Choe HH, Benian GM. |
| タイトル | Twitchin kinase interacts with MAPKAP kinase 2 in Caenorhabditis elegans striated muscle. |
| ジャーナル | Mol Biol Cell |
| Abstract |
In Caenorhabditis elegans, twitchin is a giant polypeptide located in muscle A-bands. The protein kinase of twitchin is autoinhibited by 45 residues upstream (NL) and 60 residues downstream (CRD) of the kinase catalytic core. Molecular dynamics simulation on a twitchin fragment revealed that the NL is released by pulling force. However, it is unclear how the CRD is removed. To identify proteins that may remove the CRD, we performed a yeast two-hybrid screen using twitchin kinase as bait. One interactor is MAK-1, C. elegans orthologue of MAPKAP kinase 2. MAPKAP kinase 2 is phosphorylated and activated by p38 MAP kinase. We demonstrate that the CRD of twitchin is important for binding to MAK-1. mak-1 is expressed in nematode body wall muscle, and antibodies to MAK-1 localize between and around Z-disk analogues and to the edge of A-bands. Whereas unc-22 mutants are completely resistant, mak-1 mutants are partially resistant to nicotine. MAK-1 can phosphorylate twitchin NL-Kin-CRD in vitro. Genetic data suggest the involvement of two other mak-1 paralogues and two orthologues of p38 MAP kinase. These results suggest that MAK-1 is an activator of twitchin kinase and that the p38 MAP kinase pathway may be involved in the regulation of twitchin. |
| 巻・号 | 26(11) |
| ページ | 2096-111 |
| 公開日 | 2015-6-1 |
| DOI | 10.1091/mbc.E14-05-1009 |
| PII | mbc.E14-05-1009 |
| PMID | 25851606 |
| PMC | PMC4472019 |
| MeSH | Amino Acid Motifs Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Calmodulin-Binding Proteins / metabolism* Intracellular Signaling Peptides and Proteins / metabolism* Muscle Proteins / metabolism* Muscle, Striated / enzymology* Phosphorylation Protein Binding Protein Serine-Threonine Kinases / metabolism* Sequence Homology, Amino Acid p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 3.791 |
| 引用数 | 4 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3455 tm4266 |