Reference - Detail
| RRC ID | 45621 |
|---|---|
| Author | Nelson CR, Hwang T, Chen PH, Bhalla N. |
| Title | TRIP13PCH-2 promotes Mad2 localization to unattached kinetochores in the spindle checkpoint response. |
| Journal | J Cell Biol |
| Abstract |
The spindle checkpoint acts during cell division to prevent aneuploidy, a hallmark of cancer. During checkpoint activation, Mad1 recruits Mad2 to kinetochores to generate a signal that delays anaphase onset. Yet, whether additional factors contribute to Mad2's kinetochore localization remains unclear. Here, we report that the conserved AAA+ ATPase TRIP13(PCH-2) localizes to unattached kinetochores and is required for spindle checkpoint activation in Caenorhabditis elegans. pch-2 mutants effectively localized Mad1 to unattached kinetochores, but Mad2 recruitment was significantly reduced. Furthermore, we show that the C. elegans orthologue of the Mad2 inhibitor p31(comet)(CMT-1) interacts with TRIP13(PCH-2) and is required for its localization to unattached kinetochores. These factors also genetically interact, as loss of p31(comet)(CMT-1) partially suppressed the requirement for TRIP13(PCH-2) in Mad2 localization and spindle checkpoint signaling. These data support a model in which the ability of TRIP13(PCH-2) to disassemble a p31(comet)/Mad2 complex, which has been well characterized in the context of checkpoint silencing, is also critical for spindle checkpoint activation. |
| Volume | 211(3) |
| Pages | 503-16 |
| Published | 2015-11-9 |
| DOI | 10.1083/jcb.201505114 |
| PII | jcb.201505114 |
| PMID | 26527744 |
| PMC | PMC4639874 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Adenosine Triphosphatases / metabolism Animal Husbandry / methods Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism* Cell Cycle Checkpoints / physiology* Cell Cycle Proteins / metabolism* Kinetochores / metabolism* Mad2 Proteins / metabolism* Nuclear Proteins / metabolism Signal Transduction / physiology Spindle Apparatus / metabolism* |
| IF | 8.811 |
| Times Cited | 18 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1458 |