Reference - Detail
| RRC ID | 45671 |
|---|---|
| Author | Ackema KB, Hench J, Böckler S, Wang SC, Sauder U, Mergentaler H, Westermann B, Bard F, Frank S, Spang A. |
| Title | The small GTPase Arf1 modulates mitochondrial morphology and function. |
| Journal | EMBO J |
| Abstract |
The small GTPase Arf1 plays critical roles in membrane traffic by initiating the recruitment of coat proteins and by modulating the activity of lipid-modifying enzymes. Here, we report an unexpected but evolutionarily conserved role for Arf1 and the ArfGEF GBF1 at mitochondria. Loss of function of ARF-1 or GBF-1 impaired mitochondrial morphology and activity in Caenorhabditis elegans. Similarly, mitochondrial defects were observed in mammalian and yeast cells. In Saccharomyces cerevisiae, aberrant clusters of the mitofusin Fzo1 accumulated in arf1-11 mutants and were resolved by overexpression of Cdc48, an AAA-ATPase involved in ER and mitochondria-associated degradation processes. Yeast Arf1 co-fractionated with ER and mitochondrial membranes and interacted genetically with the contact site component Gem1. Furthermore, similar mitochondrial abnormalities resulted from knockdown of either GBF-1 or contact site components in worms, suggesting that the role of Arf1 in mitochondrial functioning is linked to ER-mitochondrial contacts. Thus, Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic. |
| Volume | 33(22) |
| Pages | 2659-75 |
| Published | 2014-11-18 |
| DOI | 10.15252/embj.201489039 |
| PII | embj.201489039 |
| PMID | 25190516 |
| PMC | PMC4282574 |
| MeSH | ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mitochondria / enzymology* Mitochondria / genetics Mitochondrial Membranes / enzymology Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism |
| IF | 9.889 |
| Times Cited | 38 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1108 tm1133 |