RRC ID 45672
著者 Ahn JM, Eom HJ, Yang X, Meyer JN, Choi J.
タイトル Comparative toxicity of silver nanoparticles on oxidative stress and DNA damage in the nematode, Caenorhabditis elegans.
ジャーナル Chemosphere
Abstract This study examined the effects of polyvinylpyrrolidone (PVP) surface coating and size on the organismal and molecular toxicity of silver nanoparticles (AgNPs) on the nematode, Caenorhabditis elegans. The toxicity of bare AgNPs and 8 and 38 nm PVP-coated AgNPs (PVP8-AgNPs, PVP38-AgNPs) were compared. The toxicity of AgNO3 was also tested because ion dissolution and particle-specific effects are often important characteristics determining Ag nanotoxicity. Comparative toxicity across AgNO3 and the three different types of AgNPs was first evaluated using a C. elegans mortality test by a direct comparison of the LC50 values. Subsequently, mutant screening followed by oxidative stress, mitochondrial toxicity and DNA damage assays were carried out at equitoxic (LC10 and LC50) concentrations to further assess the toxicity mechanism of AgNO3 and AgNPs. AgNO3 and bare AgNPs had similar toxicities, whereas PVP coating reduced the toxicity of the AgNPs significantly. Of the PVP-AgNPs, the smaller NPs were more toxic. Different groups of mutants responded differently to AgNO3 and AgNPs, which indicates that their toxicity mechanism might be different. AgNO3 and bare AgNPs induced mitochondrial membrane damage. None of the silver materials tested caused detectable polymerase-inhibiting DNA lesions in either the nucleus or mitochondria as measured by a quantitative PCR assay, but AgNO3, bare AgNPs and PVP8-AgNPs induced oxidative DNA damage. These results show that coatings on the AgNPs surface and the particle size make a clear contribution to the toxicity of the AgNPs, and oxidative stress-related mitochondrial and DNA damage appear to be potential mechanisms of toxicity.
巻・号 108
ページ 343-52
公開日 2014-8-1
DOI 10.1016/j.chemosphere.2014.01.078
PII S0045-6535(14)00169-6
PMID 24726479
MeSH Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism DNA Damage / drug effects* DNA, Mitochondrial / metabolism Metal Nanoparticles / chemistry Metal Nanoparticles / toxicity* Metal Nanoparticles / ultrastructure Oxidation-Reduction Oxidative Stress / drug effects Particle Size Povidone / chemistry Povidone / toxicity* Silver / chemistry Silver / toxicity* Silver Nitrate / chemistry Silver Nitrate / toxicity*
IF 5.778
引用数 65
WOS 分野 ENVIRONMENTAL SCIENCES
リソース情報
線虫 NA