| RRC ID |
45674
|
| Author |
Alriyami MZ, Jones MR, Johnsen RC, Banerjee Y, Baillie DL.
|
| Title |
let-65 is cytoplasmic methionyl tRNA synthetase in C. elegans.
|
| Journal |
Meta Gene
|
| Abstract |
Cytoplasmic methionyl tRNA synthetase (MetRS) is one of more than 20 cytoplasmic aminoacyl tRNA synthetase enzymes (ARS). This family of enzymes catalyzes a process fundamental for protein translation. Using a combination of genetic mapping, oligonucleotide array comparative genomic hybridization, and phenotypic correlation, we show that mutations in the essential gene, let-65, reside within the predicted Caenorhabditis elegans homologue of MetRS, which we have named mars-1. We demonstrate that the lethality associated with alleles of let-65 is fully rescued by a transgenic array that spans the mars-1 genomic region. Furthermore, sequence analysis reveals that six let-65 alleles lead to the alteration of highly conserved amino acids.
|
| Volume |
2
|
| Pages |
819-30
|
| Published |
2014-12-1
|
| DOI |
10.1016/j.mgene.2014.08.006
|
| PII |
S2214-5400(14)00069-3
|
| PMID |
25606464
|
| PMC |
PMC4287814
|
| Times Cited |
0
|
| Resource |
| C.elegans |
tm3799
tm4074
tm5774
tm524
tm3947 |
