| RRC ID |
45681
|
| 著者 |
Askjaer P, Galy V, Meister P.
|
| タイトル |
Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.
|
| ジャーナル |
Methods Cell Biol
|
| Abstract |
The nematode Caenorhabditis elegans is characterized by many features that make it highly attractive to study nuclear pore complexes (NPCs) and nucleocytoplasmic transport. NPC composition and structure are highly conserved in nematodes and being amenable to a variety of genetic manipulations, key aspects of nuclear envelope dynamics can be observed in great details during breakdown, reassembly, and interphase. In this chapter, we provide an overview of some of the most relevant modern techniques that allow researchers unfamiliar with C. elegans to embark on studies of nucleoporins in an intact organism through its development from zygote to aging adult. We focus on methods relevant to generate loss-of-function phenotypes and their analysis by advanced microscopy. Extensive references to available reagents, such as mutants, transgenic strains, and antibodies are equally useful to scientists with or without prior C. elegans or nucleoporin experience.
|
| 巻・号 |
122
|
| ページ |
277-310
|
| 公開日 |
2014-1-1
|
| DOI |
10.1016/B978-0-12-417160-2.00013-8
|
| PII |
B978-0-12-417160-2.00013-8
|
| PMID |
24857735
|
| MeSH |
Active Transport, Cell Nucleus
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / metabolism*
Chromatin / metabolism
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology
Fluorescent Antibody Technique / methods
Microscopy, Electron, Transmission / methods
Nuclear Pore / metabolism*
Nuclear Pore Complex Proteins / analysis*
RNA Interference
RNA, Small Interfering
|
| IF |
1.441
|
| 引用数 |
6
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2199
tm3039
tm4329
tm2513
tm2320
tm1596
tm2784
tm2796
tm2886
tm1541
tm2952
tm1845
tm2434
tm6328
tm1437
tm1889
tm5197
tm3590
tm1439 |
