| RRC ID |
45681
|
| Author |
Askjaer P, Galy V, Meister P.
|
| Title |
Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.
|
| Journal |
Methods Cell Biol
|
| Abstract |
The nematode Caenorhabditis elegans is characterized by many features that make it highly attractive to study nuclear pore complexes (NPCs) and nucleocytoplasmic transport. NPC composition and structure are highly conserved in nematodes and being amenable to a variety of genetic manipulations, key aspects of nuclear envelope dynamics can be observed in great details during breakdown, reassembly, and interphase. In this chapter, we provide an overview of some of the most relevant modern techniques that allow researchers unfamiliar with C. elegans to embark on studies of nucleoporins in an intact organism through its development from zygote to aging adult. We focus on methods relevant to generate loss-of-function phenotypes and their analysis by advanced microscopy. Extensive references to available reagents, such as mutants, transgenic strains, and antibodies are equally useful to scientists with or without prior C. elegans or nucleoporin experience.
|
| Volume |
122
|
| Pages |
277-310
|
| Published |
2014-1-1
|
| DOI |
10.1016/B978-0-12-417160-2.00013-8
|
| PII |
B978-0-12-417160-2.00013-8
|
| PMID |
24857735
|
| MeSH |
Active Transport, Cell Nucleus
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / metabolism*
Chromatin / metabolism
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology
Fluorescent Antibody Technique / methods
Microscopy, Electron, Transmission / methods
Nuclear Pore / metabolism*
Nuclear Pore Complex Proteins / analysis*
RNA Interference
RNA, Small Interfering
|
| IF |
1.441
|
| Times Cited |
6
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm2199
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tm2784
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tm1845
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tm1439 |
