RRC ID 45769
Author Kessler Z, Yanowitz J.
Title Methodological considerations for mutagen exposure in C. elegans.
Journal Methods
Abstract Maintenance of the genome requires the continual repair of DNA lesions. Exposure of nematodes to DNA damage-inducing agents is a powerful method to rapidly ascribe a role for specific genes in DNA repair and to define epistatic relationships to other repair genes which allows for the construction of repair pathways. Despite the extensive use of these agents, however, differences in dosing, timing, and handling makes it difficult to compare results across laboratories. We provide herein a consideration of the parameters that influence the results of these exposures and detailed protocols for the exposure to mutagenic inducing agents.
Volume 68(3)
Pages 441-9
Published 2014-8-1
DOI 10.1016/j.ymeth.2014.04.011
PII S1046-2023(14)00155-8
PMID 24768858
PMC PMC5449201
MeSH Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics* DNA Damage / drug effects* DNA Repair / drug effects Genome / drug effects* Mutagens / toxicity*
IF 3.998
Times Cited 2
WOS Category BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm2026 tm2134 tm1145 tm1298 tm1866 tm2644 tm423 tm2181