論文 - 詳細
| RRC ID | 45770 |
|---|---|
| 著者 | Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC. |
| タイトル | A co-CRISPR strategy for efficient genome editing in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
Genome editing based on CRISPR (clustered regularly interspaced short palindromic repeats)-associated nuclease (Cas9) has been successfully applied in dozens of diverse plant and animal species, including the nematode Caenorhabditis elegans. The rapid life cycle and easy access to the ovary by micro-injection make C. elegans an ideal organism both for applying CRISPR-Cas9 genome editing technology and for optimizing genome-editing protocols. Here we report efficient and straightforward CRISPR-Cas9 genome-editing methods for C. elegans, including a Co-CRISPR strategy that facilitates detection of genome-editing events. We describe methods for detecting homologous recombination (HR) events, including direct screening methods as well as new selection/counterselection strategies. Our findings reveal a surprisingly high frequency of HR-mediated gene conversion, making it possible to rapidly and precisely edit the C. elegans genome both with and without the use of co-inserted marker genes. |
| 巻・号 | 197(4) |
| ページ | 1069-80 |
| 公開日 | 2014-8-1 |
| DOI | 10.1534/genetics.114.166389 |
| PII | genetics.114.166389 |
| PMID | 24879462 |
| PMC | PMC4125384 |
| MeSH | Animals Base Sequence CRISPR-Associated Proteins / genetics* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Deoxyribonucleases / genetics* Genetic Markers Genome, Helminth* Homologous Recombination / genetics Molecular Sequence Data Mutagenesis, Insertional Plasmids / genetics Sequence Analysis, DNA |
| IF | 4.015 |
| 引用数 | 120 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1396 |