論文 - 詳細
| RRC ID | 45781 |
|---|---|
| 著者 | Lant B, Yu B, Goudreault M, Holmyard D, Knight JD, Xu P, Zhao L, Chin K, Wallace E, Zhen M, Gingras AC, Derry WB. |
| タイトル | CCM-3/STRIPAK promotes seamless tube extension through endocytic recycling. |
| ジャーナル | Nat Commun |
| Abstract |
The mechanisms governing apical membrane assembly during biological tube development are poorly understood. Here, we show that extension of the C. elegans excretory canal requires cerebral cavernous malformation 3 (CCM-3), independent of the CCM1 orthologue KRI-1. Loss of ccm-3 causes canal truncations and aggregations of canaliculular vesicles, which form ectopic lumen (cysts). We show that CCM-3 localizes to the apical membrane, and in cooperation with GCK-1 and STRIPAK, promotes CDC-42 signalling, Golgi stability and endocytic recycling. We propose that endocytic recycling is mediated through the CDC-42-binding kinase MRCK-1, which interacts physically with CCM-3-STRIPAK. We further show canal membrane integrity to be dependent on the exocyst complex and the actin cytoskeleton. This work reveals novel in vivo roles of CCM-3·STRIPAK in regulating tube extension and membrane integrity through small GTPase signalling and vesicle dynamics, which may help explain the severity of CCM3 mutations in patients. |
| 巻・号 | 6 |
| ページ | 6449 |
| 公開日 | 2015-3-6 |
| DOI | 10.1038/ncomms7449 |
| PII | ncomms7449 |
| PMID | 25743393 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* GTP-Binding Proteins / metabolism* Golgi Apparatus / metabolism Intellectual Disability / metabolism* Intestines / growth & development Micrognathism / metabolism* Microscopy, Electron, Transmission Microscopy, Interference Morphogenesis / physiology* RNA Interference Ribs / abnormalities* Ribs / metabolism Signal Transduction / physiology* Transport Vesicles / physiology* |
| IF | 12.121 |
| 引用数 | 35 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2806 |