Reference - Detail
| RRC ID | 45832 |
|---|---|
| Author | Pukkila-Worley R, Feinbaum RL, McEwan DL, Conery AL, Ausubel FM. |
| Title | The evolutionarily conserved mediator subunit MDT-15/MED15 links protective innate immune responses and xenobiotic detoxification. |
| Journal | PLoS Pathog |
| Abstract |
Metazoans protect themselves from environmental toxins and virulent pathogens through detoxification and immune responses. We previously identified a small molecule xenobiotic toxin that extends survival of Caenorhabditis elegans infected with human bacterial pathogens by activating the conserved p38 MAP kinase PMK-1 host defense pathway. Here we investigate the cellular mechanisms that couple activation of a detoxification response to innate immunity. From an RNAi screen of 1,420 genes expressed in the C. elegans intestine, we identified the conserved Mediator subunit MDT-15/MED15 and 28 other gene inactivations that abrogate the induction of PMK-1-dependent immune effectors by this small molecule. We demonstrate that MDT-15/MED15 is required for the xenobiotic-induced expression of p38 MAP kinase PMK-1-dependent immune genes and protection from Pseudomonas aeruginosa infection. We also show that MDT-15 controls the induction of detoxification genes and functions to protect the host from bacteria-derived phenazine toxins. These data define a central role for MDT-15/MED15 in the coordination of xenobiotic detoxification and innate immune responses. |
| Volume | 10(5) |
| Pages | e1004143 |
| Published | 2014-5-1 |
| DOI | 10.1371/journal.ppat.1004143 |
| PII | PPATHOGENS-D-13-03022 |
| PMID | 24875643 |
| PMC | PMC4038581 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / genetics* Evolution, Molecular* Immunity, Innate / genetics* MAP Kinase Signaling System / physiology Pseudomonas aeruginosa* / genetics RNA Interference Transcription Factors / genetics* |
| IF | 6.218 |
| Times Cited | 24 |
| WOS Category | PARASITOLOGY MICROBIOLOGY VIROLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2182 |