論文 - 詳細
| RRC ID | 45856 |
|---|---|
| 著者 | Shirayama M, Stanney W 3rd, Gu W, Seth M, Mello CC. |
| タイトル | The Vasa Homolog RDE-12 engages target mRNA and multiple argonaute proteins to promote RNAi in C. elegans. |
| ジャーナル | Curr Biol |
| Abstract |
Argonaute (AGO) proteins are key nuclease effectors of RNAi. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, which engages amplified small RNAs during RNAi. These studies identified a robust association between WAGO-1 and a conserved Vasa ATPase-related protein RDE-12. rde-12 mutants are deficient in RNAi, including viral suppression, and fail to produce amplified secondary siRNAs and certain endogenous siRNAs (endo-siRNAs). RDE-12 colocalizes with WAGO-1 in germline P granules and in cytoplasmic and perinuclear foci in somatic cells. These findings and our genetic studies suggest that RDE-12 is first recruited to target mRNA by upstream AGOs (RDE-1 and ERGO-1), where it promotes small RNA amplification and/or WAGO-1 loading. Downstream of these events, RDE-12 forms an RNase-resistant (target mRNA-independent) complex with WAGO-1 and may thus have additional functions in target mRNA surveillance and silencing. |
| 巻・号 | 24(8) |
| ページ | 845-51 |
| 公開日 | 2014-4-14 |
| DOI | 10.1016/j.cub.2014.03.008 |
| PII | S0960-9822(14)00267-X |
| PMID | 24684931 |
| PMC | PMC4017897 |
| MeSH | Amino Acid Sequence Animals Argonaute Proteins / metabolism* Blotting, Northern Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Immunoblotting Immunoprecipitation Microscopy, Fluorescence Molecular Sequence Data RNA Interference / physiology* RNA, Messenger / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* |
| IF | 9.601 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3644 tm3679 |