論文 - 詳細
| RRC ID | 45884 |
|---|---|
| 著者 | Wohlschlager T, Butschi A, Grassi P, Sutov G, Gauss R, Hauck D, Schmieder SS, Knobel M, Titz A, Dell A, Haslam SM, Hengartner MO, Aebi M, Künzler M. |
| タイトル | Methylated glycans as conserved targets of animal and fungal innate defense. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Effector proteins of innate immune systems recognize specific non-self epitopes. Tectonins are a family of β-propeller lectins conserved from bacteria to mammals that have been shown to bind bacterial lipopolysaccharide (LPS). We present experimental evidence that two Tectonins of fungal and animal origin have a specificity for O-methylated glycans. We show that Tectonin 2 of the mushroom Laccaria bicolor (Lb-Tec2) agglutinates Gram-negative bacteria and exerts toxicity toward the model nematode Caenorhabditis elegans, suggesting a role in fungal defense against bacteria and nematodes. Biochemical and genetic analysis of these interactions revealed that both bacterial agglutination and nematotoxicity of Lb-Tec2 depend on the recognition of methylated glycans, namely O-methylated mannose and fucose residues, as part of bacterial LPS and nematode cell-surface glycans. In addition, a C. elegans gene, termed samt-1, coding for a candidate membrane transport protein for the presumptive donor substrate of glycan methylation, S-adenosyl-methionine, from the cytoplasm to the Golgi was identified. Intriguingly, limulus lectin L6, a structurally related antibacterial protein of the Japanese horseshoe crab Tachypleus tridentatus, showed properties identical to the mushroom lectin. These results suggest that O-methylated glycans constitute a conserved target of the fungal and animal innate immune system. The broad phylogenetic distribution of O-methylated glycans increases the spectrum of potential antagonists recognized by Tectonins, rendering this conserved protein family a universal defense armor. |
| 巻・号 | 111(27) |
| ページ | E2787-96 |
| 公開日 | 2014-7-8 |
| DOI | 10.1073/pnas.1401176111 |
| PII | 1401176111 |
| PMID | 24879441 |
| PMC | PMC4103367 |
| MeSH | Agaricales / immunology* Amino Acid Sequence Animals Caenorhabditis elegans / immunology Horseshoe Crabs / immunology Immunity, Innate* Membrane Proteins / chemistry Membrane Proteins / metabolism Methylation Molecular Sequence Data Phylogeny Polysaccharides / metabolism* Sequence Homology, Amino Acid |
| IF | 9.412 |
| 引用数 | 39 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1078 tm2725 tm2530 |