論文 - 詳細
| RRC ID | 4589 |
|---|---|
| 著者 | Mukai H, Hokari Y, Seki T, Takao T, Kubota M, Matsuo Y, Tsukagoshi H, Kato M, Kimura H, Shimonishi Y, Kiso Y, Nishi Y, Wakamatsu K, Munekata E. |
| タイトル | Discovery of mitocryptide-1, a neutrophil-activating cryptide from healthy porcine heart. |
| ジャーナル | J Biol Chem |
| Abstract |
Although neutrophils are known to migrate in response to various chemokines and complement factors, the substances involved in the early stages of their transmigration and activation have been poorly characterized to date. Here we report the discovery of a peptide isolated from healthy porcine hearts that activated neutrophils. Its primary structure is H-Leu-Ser-Phe-Leu-Ile-Pro-Ala-Gly-Trp-Val-Leu-Ser-His-Leu-Asp-His-Tyr-Lys-Arg-Ser-Ser-Ala-Ala-OH, and it was indicated to originate from mitochondrial cytochrome c oxidase subunit VIII. This peptide caused chemotaxis at concentrations lower than that inducing beta-hexosaminidase release. Such responses were observed in neutrophilic/granulocytic differentiated HL-60 cells but not in undifferentiated cells, and G(i2)-type G proteins were suggested to be involved in the peptide signaling. Moreover the peptide activated human neutrophils to induce beta-hexosaminidase secretion. A number of other amphipathic neutrophil-activating peptides presumably originating from mitochondrial proteins were also found. The present results suggest that neutrophils monitor such amphipathic peptides including the identified peptide as an initiation signal for inflammation at injury sites. |
| 巻・号 | 283(45) |
| ページ | 30596-605 |
| 公開日 | 2008-11-7 |
| DOI | 10.1074/jbc.M803913200 |
| PII | S0021-9258(20)64631-2 |
| PMID | 18768476 |
| PMC | PMC2662150 |
| MeSH | Animals Chemotaxis / drug effects Electron Transport Complex IV / chemistry Electron Transport Complex IV / isolation & purification* Electron Transport Complex IV / pharmacology HL-60 Cells Humans Mitochondrial Proteins / chemistry Mitochondrial Proteins / isolation & purification* Mitochondrial Proteins / pharmacology Muscle Proteins / chemistry Muscle Proteins / isolation & purification* Muscle Proteins / pharmacology Myocardium / chemistry* Neutrophil Activation / drug effects* Neutrophils / metabolism* Peptides / chemistry Peptides / isolation & purification* Peptides / pharmacology Swine beta-N-Acetylhexosaminidases / metabolism |
| IF | 4.238 |
| 引用数 | 27 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |