Reference - Detail
| RRC ID | 45939 |
|---|---|
| Author | Castellano-Pozo M, Santos-Pereira JM, Rondón AG, Barroso S, Andújar E, Pérez-Alegre M, García-Muse T, Aguilera A. |
| Title | R loops are linked to histone H3 S10 phosphorylation and chromatin condensation. |
| Journal | Mol Cell |
| Abstract |
R loops are transcription byproducts that constitute a threat to genome integrity. Here we show that R loops are tightly linked to histone H3 S10 phosphorylation (H3S10P), a mark of chromatin condensation. Chromatin immunoprecipitation (ChIP)-on-chip (ChIP-chip) analyses reveal H3S10P accumulation at centromeres, pericentromeric chromatin, and a large number of active open reading frames (ORFs) in R-loop-accumulating yeast cells, better observed in G1. Histone H3S10 plays a key role in maintaining genome stability, as scored by ectopic recombination and plasmid loss, Rad52 foci, and Rad53 checkpoint activation. H3S10P coincides with the presence of DNA-RNA hybrids, is suppressed by ribonuclease H overexpression, and causes reduced accessibility of restriction endonucleases, implying a tight connection between R loops, H3S10P, and chromatin compaction. Such histone modifications were also observed in R-loop-accumulating Caenorhabditis elegans and HeLa cells. We therefore provide a role of RNA in chromatin structure essential to understand how R loops modulate genome dynamics. |
| Volume | 52(4) |
| Pages | 583-90 |
| Published | 2013-11-21 |
| DOI | 10.1016/j.molcel.2013.10.006 |
| PII | S1097-2765(13)00747-8 |
| PMID | 24211264 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / metabolism* Chromatin Assembly and Disassembly Chromatin Immunoprecipitation DNA, Single-Stranded / genetics* Genomic Instability HeLa Cells Histones / metabolism* Humans Meiosis Mitosis Open Reading Frames Phosphorylation Protein Processing, Post-Translational* RNA Polymerase II / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / metabolism* Transcription, Genetic |
| IF | 15.584 |
| Times Cited | 126 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1310 |