論文 - 詳細
| RRC ID | 45976 |
|---|---|
| 著者 | Gomes JE, Tavernier N, Richaudeau B, Formstecher E, Boulin T, Mains PE, Dumont J, Pintard L. |
| タイトル | Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation. |
| ジャーナル | J Cell Biol |
| Abstract |
Katanin is an evolutionarily conserved microtubule (MT)-severing complex implicated in multiple aspects of MT dynamics. In Caenorhabditis elegans, the katanin homologue MEI-1 is required for meiosis, but must be inactivated before mitosis. Here we show that PPFR-1, a regulatory subunit of a trimeric protein phosphatase 4 complex, enhanced katanin MT-severing activity during C. elegans meiosis. Loss of ppfr-1, similarly to the inactivation of MT severing, caused a specific defect in meiosis II spindle disassembly. We show that a fraction of PPFR-1 was degraded after meiosis, contributing to katanin inactivation. PPFR-1 interacted with MEL-26, the substrate recognition subunit of the CUL-3 RING E3 ligase (CRL3(MEL-26)), which also targeted MEI-1 for post-meiotic degradation. Reversible protein phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis. |
| 巻・号 | 202(3) |
| ページ | 431-9 |
| 公開日 | 2013-8-5 |
| DOI | 10.1083/jcb.201304174 |
| PII | jcb.201304174 |
| PMID | 23918937 |
| PMC | PMC3734088 |
| MeSH | Adenosine Triphosphatases / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Katanin Microtubules / metabolism* Multiprotein Complexes / metabolism Phosphoprotein Phosphatases / metabolism* Phosphorylation |
| IF | 8.811 |
| 引用数 | 9 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1664 tm2180 |