RRC ID 46010
Author Alan JK, Lundquist EA.
Title Analysis of Rho GTPase function in axon pathfinding using Caenorhabditis elegans.
Journal Methods Mol. Biol.
Abstract We provide information and protocols for the analysis of Rho GTPase function in axon pathfinding in Caenorhabditis elegans. The powerful molecular, genetic, imaging, and transgenic tools available in C. elegans make it an excellent system in which to study the in vivo roles of Rho GTPases. Methods for imaging of axon morphology in Rho GTPase single and double mutants are provided, as well as methods for the construction of transgenic C. elegans strains carrying exogenously introduced transgenes that drive the expression of constitutively active and dominant negative mutants.
Volume 827
Pages 339-58
Published 2012
DOI 10.1007/978-1-61779-442-1_22
PMID 22144285
MeSH Animals Animals, Genetically Modified Axons / enzymology* Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Female Male Microscopy, Fluorescence Neurons / cytology Neurons / metabolism Transgenes rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism*
Times Cited 2
WOS Category CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm597