論文 - 詳細
RRC ID | 46029 |
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著者 | Blum ES, Abraham MC, Yoshimura S, Lu Y, Shaham S. |
タイトル | Control of nonapoptotic developmental cell death in Caenorhabditis elegans by a polyglutamine-repeat protein. |
ジャーナル | Science |
Abstract |
Death is a vital developmental cell fate. In Caenorhabditis elegans, programmed death of the linker cell, which leads gonadal elongation, proceeds independently of caspases and apoptotic effectors. To identify genes promoting linker-cell death, we performed a genome-wide RNA interference screen. We show that linker-cell death requires the gene pqn-41, encoding an endogenous polyglutamine-repeat protein. pqn-41 functions cell-autonomously and is expressed at the onset of linker-cell death. pqn-41 expression is controlled by the mitogen-activated protein kinase kinase SEK-1, which functions in parallel to the zinc-finger protein LIN-29 to promote cellular demise. Linker-cell death is morphologically similar to cell death associated with normal vertebrate development and polyglutamine-induced neurodegeneration. Our results may therefore provide molecular inroads to understanding nonapoptotic cell death in metazoan development and disease. |
巻・号 | 335(6071) |
ページ | 970-3 |
公開日 | 2012-2-24 |
DOI | 10.1126/science.1215156 |
PII | 335/6071/970 |
PMID | 22363008 |
PMC | PMC3858082 |
MeSH | Alleles Amino Acid Motifs Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Death* Cell Nucleus / ultrastructure Cell Survival DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation Genes, Helminth Genome, Helminth MAP Kinase Kinase 4 / genetics MAP Kinase Kinase 4 / metabolism Male Molecular Sequence Data Mutant Proteins / chemistry Mutant Proteins / metabolism Peptides / chemistry Protein Structure, Tertiary RNA Interference Sequence Deletion Transcription Factors / genetics Transcription Factors / metabolism Transgenes |
IF | 41.846 |
引用数 | 55 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
線虫 | tm4024 |