RRC ID 46029
著者 Blum ES, Abraham MC, Yoshimura S, Lu Y, Shaham S.
タイトル Control of nonapoptotic developmental cell death in Caenorhabditis elegans by a polyglutamine-repeat protein.
ジャーナル Science
Abstract Death is a vital developmental cell fate. In Caenorhabditis elegans, programmed death of the linker cell, which leads gonadal elongation, proceeds independently of caspases and apoptotic effectors. To identify genes promoting linker-cell death, we performed a genome-wide RNA interference screen. We show that linker-cell death requires the gene pqn-41, encoding an endogenous polyglutamine-repeat protein. pqn-41 functions cell-autonomously and is expressed at the onset of linker-cell death. pqn-41 expression is controlled by the mitogen-activated protein kinase kinase SEK-1, which functions in parallel to the zinc-finger protein LIN-29 to promote cellular demise. Linker-cell death is morphologically similar to cell death associated with normal vertebrate development and polyglutamine-induced neurodegeneration. Our results may therefore provide molecular inroads to understanding nonapoptotic cell death in metazoan development and disease.
巻・号 335(6071)
ページ 970-3
公開日 2012-2-24
DOI 10.1126/science.1215156
PII 335/6071/970
PMID 22363008
PMC PMC3858082
MeSH Alleles Amino Acid Motifs Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Death* Cell Nucleus / ultrastructure Cell Survival DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation Genes, Helminth Genome, Helminth MAP Kinase Kinase 4 / genetics MAP Kinase Kinase 4 / metabolism Male Molecular Sequence Data Mutant Proteins / chemistry Mutant Proteins / metabolism Peptides / chemistry Protein Structure, Tertiary RNA Interference Sequence Deletion Transcription Factors / genetics Transcription Factors / metabolism Transgenes
IF 41.846
引用数 55
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm4024