RRC ID 46034
著者 Lambie EJ, Tieu PJ, Lebedeva N, Church DL, Conradt B.
タイトル CATP-6, a C. elegans ortholog of ATP13A2 PARK9, positively regulates GEM-1, an SLC16A transporter.
ジャーナル PLoS One
Abstract In previous work, we found that gain-of-function mutations that hyperactivate GEM-1 (an SLC16A transporter protein) can bypass the requirement for GON-2 (a TRPM channel protein) during the initiation of gonadogenesis in C. elegans. Consequently, we proposed that GEM-1 might function as part of a Mg(2+) uptake pathway that functions in parallel to GON-2. In this study, we report that CATP-6, a C. elegans ortholog of the P5B ATPase, ATP13A2 (PARK9), is necessary for gem-1 gain-of-function mutations to suppress the effects of gon-2 inactivation. One possible explanation for this observation is that GEM-1 serves to activate CATP-6, which then functions as a Mg(2+) transporter. However, we found that overexpression of GEM-1 can alleviate the requirement for CATP-6 activity, suggesting that CATP-6 probably acts as a non-essential upstream positive regulator of GEM-1. Our results are consistent with the notion that P5B ATPases govern intracellular levels of Mg(2+) and/or Mn(2+) by regulating the trafficking of transporters and other proteins associated with the plasma membrane.
巻・号 8(10)
ページ e77202
公開日 2013-1-1
DOI 10.1371/journal.pone.0077202
PII PONE-D-13-32840
PMID 24130856
PMC PMC3793975
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Amino Acid Sequence Animals Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental Genotype Gonads / growth & development Magnesium / metabolism Molecular Sequence Data Monocarboxylic Acid Transporters / genetics* Monocarboxylic Acid Transporters / metabolism* Proton-Translocating ATPases / chemistry* Sequence Homology* Suppression, Genetic
IF 2.74
引用数 5
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm3190