論文 - 詳細
RRC ID | 46034 |
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著者 | Lambie EJ, Tieu PJ, Lebedeva N, Church DL, Conradt B. |
タイトル | CATP-6, a C. elegans ortholog of ATP13A2 PARK9, positively regulates GEM-1, an SLC16A transporter. |
ジャーナル | PLoS One |
Abstract |
In previous work, we found that gain-of-function mutations that hyperactivate GEM-1 (an SLC16A transporter protein) can bypass the requirement for GON-2 (a TRPM channel protein) during the initiation of gonadogenesis in C. elegans. Consequently, we proposed that GEM-1 might function as part of a Mg(2+) uptake pathway that functions in parallel to GON-2. In this study, we report that CATP-6, a C. elegans ortholog of the P5B ATPase, ATP13A2 (PARK9), is necessary for gem-1 gain-of-function mutations to suppress the effects of gon-2 inactivation. One possible explanation for this observation is that GEM-1 serves to activate CATP-6, which then functions as a Mg(2+) transporter. However, we found that overexpression of GEM-1 can alleviate the requirement for CATP-6 activity, suggesting that CATP-6 probably acts as a non-essential upstream positive regulator of GEM-1. Our results are consistent with the notion that P5B ATPases govern intracellular levels of Mg(2+) and/or Mn(2+) by regulating the trafficking of transporters and other proteins associated with the plasma membrane. |
巻・号 | 8(10) |
ページ | e77202 |
公開日 | 2013-1-1 |
DOI | 10.1371/journal.pone.0077202 |
PII | PONE-D-13-32840 |
PMID | 24130856 |
PMC | PMC3793975 |
MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Amino Acid Sequence Animals Caenorhabditis elegans / chemistry* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation, Developmental Genotype Gonads / growth & development Magnesium / metabolism Molecular Sequence Data Monocarboxylic Acid Transporters / genetics* Monocarboxylic Acid Transporters / metabolism* Proton-Translocating ATPases / chemistry* Sequence Homology* Suppression, Genetic |
IF | 2.74 |
引用数 | 5 |
WOS 分野 | GENETICS & HEREDITY |
リソース情報 | |
線虫 | tm3190 |