論文 - 詳細
| RRC ID | 46094 |
|---|---|
| 著者 | Franz A, Orth M, Pirson PA, Sonneville R, Blow JJ, Gartner A, Stemmann O, Hoppe T. |
| タイトル | CDC-48/p97 coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication. |
| ジャーナル | Mol Cell |
| Abstract |
Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, the CDC-45/GINS complex moves with the replication fork; however, it is largely unknown how its chromatin association is regulated. Here, we show that the chaperone-like ATPase CDC-48/p97 coordinates degradation of CDT-1 with release of the CDC-45/GINS complex. C. elegans embryos lacking CDC-48 or its cofactors UFD-1/NPL-4 accumulate CDT-1 on mitotic chromatin, indicating a critical role of CDC-48 in CDT-1 turnover. Strikingly, CDC-48(UFD-1/NPL-4)-deficient embryos show persistent chromatin association of CDC-45/GINS, which is a consequence of CDT-1 stabilization. Moreover, our data confirmed a similar regulation in Xenopus egg extracts, emphasizing a conserved coordination of licensing and elongation events during eukaryotic DNA replication by CDC-48/p97. |
| 巻・号 | 44(1) |
| ページ | 85-96 |
| 公開日 | 2011-10-7 |
| DOI | 10.1016/j.molcel.2011.08.028 |
| PII | S1097-2765(11)00687-3 |
| PMID | 21981920 |
| PMC | PMC3428722 |
| MeSH | Adenosine Triphosphatases / metabolism* Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Chromatin / metabolism* Chromosomal Proteins, Non-Histone / metabolism* DNA Replication* Ligases / metabolism* Male Mitosis RNA Interference Spermatozoa / metabolism Two-Hybrid System Techniques Ubiquitin / chemistry Ubiquitin / metabolism Valosin Containing Protein Xenopus Proteins / metabolism* Xenopus laevis |
| IF | 15.584 |
| 引用数 | 58 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm544 |