Reference - Detail
| RRC ID | 46110 |
|---|---|
| Author | Harterink M, Port F, Lorenowicz MJ, McGough IJ, Silhankova M, Betist MC, van Weering JRT, van Heesbeen RGHP, Middelkoop TC, Basler K, Cullen PJ, Korswagen HC. |
| Title | A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion. |
| Journal | Nat Cell Biol |
| Abstract |
Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding protein Wntless (Wls), which transports Wnt from the Golgi network to the cell surface for release. It has recently been shown that recycling of Wls through a retromer-dependent endosome-to-Golgi trafficking pathway is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is poorly understood. Here, we report that Wls recycling is mediated through a retromer pathway that is independent of the retromer sorting nexins SNX1-SNX2 and SNX5-SNX6. We have found that the unrelated sorting nexin, SNX3, has an evolutionarily conserved function in Wls recycling and Wnt secretion and show that SNX3 interacts directly with the cargo-selective subcomplex of the retromer to sort Wls into a morphologically distinct retrieval pathway. These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo. |
| Volume | 13(8) |
| Pages | 914-923 |
| Published | 2011-7-3 |
| DOI | 10.1038/ncb2281 |
| PMID | 21725319 |
| PMC | PMC4052212 |
| MeSH | Animals Animals, Genetically Modified Biological Transport, Active Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Drosophila / genetics Drosophila / growth & development Drosophila / metabolism Endosomes / metabolism HeLa Cells Humans Intracellular Signaling Peptides and Proteins / metabolism* Models, Biological RNA Interference Signal Transduction Sorting Nexins / antagonists & inhibitors Sorting Nexins / genetics Sorting Nexins / metabolism* Wnt Proteins / metabolism* trans-Golgi Network / metabolism |
| IF | 20.042 |
| Times Cited | 182 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1595 tm1320 tm3790 tm1523 tm847 |