| RRC ID |
46144
|
| Author |
Kouns NA, Nakielna J, Behensky F, Krause MW, Kostrouch Z, Kostrouchova M.
|
| Title |
NHR-23 dependent collagen and hedgehog-related genes required for molting.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
NHR-23, a conserved member of the nuclear receptor family of transcription factors, is required for normal development in Caenorhabditis elegans where it plays a critical role in growth and molting. In a search for NHR-23 dependent genes, we performed whole genome comparative expression microarrays on both control and nhr-23 inhibited synchronized larvae. Genes that decreased in response to nhr-23 RNAi included several collagen genes. Unexpectedly, several hedgehog-related genes were also down-regulated after nhr-23 RNAi. A homozygous nhr-23 deletion allele was used to confirm the RNAi knockdown phenotypes and the changes in gene expression. Our results indicate that NHR-23 is a critical co-regulator of functionally linked genes involved in growth and molting and reveal evolutionary parallels among the ecdysozoa.
|
| Volume |
413(4)
|
| Pages |
515-20
|
| Published |
2011-10-7
|
| DOI |
10.1016/j.bbrc.2011.08.124
|
| PII |
S0006-291X(11)01543-9
|
| PMID |
21910973
|
| PMC |
PMC3196369
|
| MeSH |
Amino Acid Sequence
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / growth & development*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology*
Gene Deletion
Gene Expression Regulation, Developmental*
Hedgehog Proteins / metabolism*
Molecular Sequence Data
Molting / genetics*
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / physiology*
Transcription Factors / genetics
Transcription Factors / physiology*
|
| IF |
2.985
|
| Times Cited |
18
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| C.elegans |
tm1323 |
