論文 - 詳細
| RRC ID | 46236 |
|---|---|
| 著者 | Wernike D, van Oostende C, Piekny A. |
| タイトル | Visualizing neuroblast cytokinesis during C. elegans embryogenesis. |
| ジャーナル | J Vis Exp |
| Abstract |
This protocol describes the use of fluorescence microscopy to image dividing cells within developing Caenorhabditis elegans embryos. In particular, this protocol focuses on how to image dividing neuroblasts, which are found underneath the epidermal cells and may be important for epidermal morphogenesis. Tissue formation is crucial for metazoan development and relies on external cues from neighboring tissues. C. elegans is an excellent model organism to study tissue morphogenesis in vivo due to its transparency and simple organization, making its tissues easy to study via microscopy. Ventral enclosure is the process where the ventral surface of the embryo is covered by a single layer of epithelial cells. This event is thought to be facilitated by the underlying neuroblasts, which provide chemical guidance cues to mediate migration of the overlying epithelial cells. However, the neuroblasts are highly proliferative and also may act as a mechanical substrate for the ventral epidermal cells. Studies using this experimental protocol could uncover the importance of intercellular communication during tissue formation, and could be used to reveal the roles of genes involved in cell division within developing tissues. |
| 巻・号 | (85) |
| 公開日 | 2014-3-12 |
| DOI | 10.3791/51188 |
| PMID | 24686748 |
| PMC | PMC4145831 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology* Cytokinesis / physiology Embryo, Nonmammalian / cytology Microscopy, Fluorescence / methods* Neurons / cytology* |
| IF | 1.163 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4063 |