論文 - 詳細
| RRC ID | 46255 |
|---|---|
| 著者 | Ohba Y, Sakuragi T, Kage-Nakadai E, Tomioka NH, Kono N, Imae R, Inoue A, Aoki J, Ishihara N, Inoue T, Mitani S, Arai H. |
| タイトル | Mitochondria-type GPAT is required for mitochondrial fusion. |
| ジャーナル | EMBO J |
| Abstract |
Glycerol-3-phosphate acyltransferase (GPAT) is involved in the first step in glycerolipid synthesis and is localized in both the endoplasmic reticulum (ER) and mitochondria. To clarify the functional differences between ER-GPAT and mitochondrial (Mt)-GPAT, we generated both GPAT mutants in C. elegans and demonstrated that Mt-GPAT is essential for mitochondrial fusion. Mutation of Mt-GPAT caused excessive mitochondrial fragmentation. The defect was rescued by injection of lysophosphatidic acid (LPA), a direct product of GPAT, and by inhibition of LPA acyltransferase, both of which lead to accumulation of LPA in the cells. Mitochondrial fragmentation in Mt-GPAT mutants was also rescued by inhibition of mitochondrial fission protein DRP-1 and by overexpression of mitochondrial fusion protein FZO-1/mitofusin, suggesting that the fusion/fission balance is affected by Mt-GPAT depletion. Mitochondrial fragmentation was also observed in Mt-GPAT-depleted HeLa cells. A mitochondrial fusion assay using HeLa cells revealed that Mt-GPAT depletion impaired mitochondrial fusion process. We postulate from these results that LPA produced by Mt-GPAT functions not only as a precursor for glycerolipid synthesis but also as an essential factor of mitochondrial fusion. |
| 巻・号 | 32(9) |
| ページ | 1265-79 |
| 公開日 | 2013-5-2 |
| DOI | 10.1038/emboj.2013.77 |
| PII | emboj201377 |
| PMID | 23572076 |
| PMC | PMC3642685 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology Female Gene Deletion Glycerol-3-Phosphate O-Acyltransferase / genetics Glycerol-3-Phosphate O-Acyltransferase / metabolism* Glycerol-3-Phosphate O-Acyltransferase / physiology Lysophospholipids / metabolism Lysophospholipids / pharmacology Microsomes / metabolism Mitochondria / drug effects Mitochondria / enzymology* Mitochondria / genetics Mitochondrial Dynamics* Mitochondrial Size / drug effects Mitochondrial Size / genetics Models, Biological Mutagenesis, Site-Directed Oogenesis / genetics |
| IF | 9.889 |
| 引用数 | 30 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3396 tm3452 tm3289 tm3246 tm1133 |