RRC ID |
46276
|
Author |
Burger J, Merlet J, Tavernier N, Richaudeau B, Arnold A, Ciosk R, Bowerman B, Pintard L.
|
Title |
CRL2(LRR-1) E3-ligase regulates proliferation and progression through meiosis in the Caenorhabditis elegans germline.
|
Journal |
PLoS Genet
|
Abstract |
The ubiquitin-proteolytic system controls the stability of proteins in space and time. In this study, using a temperature-sensitive mutant allele of the cul-2 gene, we show that CRL2(LRR-1) (CUL-2 RING E3 ubiquitin-ligase and the Leucine Rich Repeat 1 substrate recognition subunit) acts at multiple levels to control germline development. CRL2(LRR-1) promotes germ cell proliferation by counteracting the DNA replication ATL-1 checkpoint pathway. CRL2(LRR-1) also participates in the mitotic proliferation/meiotic entry decision, presumably controlling the stability of meiotic promoting factors in the mitotic zone of the germline. Finally, CRL2(LRR-1) inhibits the first steps of meiotic prophase by targeting in mitotic germ cells degradation of the HORMA domain-containing protein HTP-3, required for loading synaptonemal complex components onto meiotic chromosomes. Given its widespread evolutionary conservation, CUL-2 may similarly regulate germline development in other organisms as well.
|
Volume |
9(3)
|
Pages |
e1003375
|
Published |
2013-3-1
|
DOI |
10.1371/journal.pgen.1003375
|
PII |
PGENETICS-D-12-01810
|
PMID |
23555289
|
PMC |
PMC3610609
|
MeSH |
Animals
Ataxia Telangiectasia Mutated Proteins
Caenorhabditis elegans* / cytology
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cell Cycle / genetics
Cell Cycle Proteins / metabolism
Cell Proliferation*
Cullin Proteins* / genetics
Cullin Proteins* / metabolism
DNA Replication
Germ Cells / cytology
Germ Cells / metabolism
Meiosis / genetics*
Mitosis
Phosphotransferases / metabolism
Synaptonemal Complex / metabolism
|
IF |
5.175
|
Times Cited |
23
|
WOS Category
|
GENETICS & HEREDITY
|
Resource |
C.elegans |
tm3543 |