論文 - 詳細
| RRC ID | 46304 |
|---|---|
| 著者 | Cox-Paulson EA, Walck-Shannon E, Lynch AM, Yamashiro S, Zaidel-Bar R, Eno CC, Ono S, Hardin J. |
| タイトル | Tropomodulin protects α-catenin-dependent junctional-actin networks under stress during epithelial morphogenesis. |
| ジャーナル | Curr Biol |
| Abstract |
α-catenin is central to recruitment of actin networks to the cadherin-catenin complex, but how such networks are subsequently stabilized against stress applied during morphogenesis is poorly understood. To identify proteins that functionally interact with α-catenin in this process, we performed enhancer screening using a weak allele of the C. elegans α-catenin, hmp-1, thereby identifying UNC-94/tropomodulin. Tropomodulins (Tmods) cap the minus ends of F-actin in sarcomeres. They also regulate lamellipodia, can promote actin nucleation, and are required for normal cardiovascular development and neuronal growth-cone morphology. Tmods regulate the morphology of cultured epithelial cells, but their role in epithelia in vivo remains unexplored. We find that UNC-94 is enriched within a HMP-1-dependent junctional-actin network at epidermal adherens junctions subject to stress during morphogenesis. Loss of UNC-94 leads to discontinuity of this network, and high-speed filming of hmp-1(fe4);unc-94(RNAi) embryos reveals large junctional displacements that depend on the Rho pathway. In vitro, UNC-94 acts in combination with HMP-1, leading to longer actin bundles than with HMP-1 alone. Our data suggest that Tmods protect actin filaments recruited by α-catenin from minus-end subunit loss, enabling them to withstand the stresses of morphogenesis. |
| 巻・号 | 22(16) |
| ページ | 1500-5 |
| 公開日 | 2012-8-21 |
| DOI | 10.1016/j.cub.2012.06.025 |
| PII | S0960-9822(12)00670-7 |
| PMID | 22771044 |
| PMC | PMC3427464 |
| MeSH | Actins / metabolism* Animals Caenorhabditis elegans Epidermis / embryology Morphogenesis* Stress, Mechanical* Tropomodulin / metabolism* alpha Catenin / metabolism* |
| IF | 9.601 |
| 引用数 | 19 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm724 |