論文 - 詳細
| RRC ID | 46316 |
|---|---|
| 著者 | Petty E, Laughlin E, Csankovszki G. |
| タイトル | Regulation of DCC localization by HTZ-1/H2A.Z and DPY-30 does not correlate with H3K4 methylation levels. |
| ジャーナル | PLoS One |
| Abstract |
Dosage compensation is a specialized form of gene regulation that balances sex-chromosome linked gene expression between the sexes. In C. elegans, dosage compensation is achieved by the activity of the dosage compensation complex (DCC). The DCC binds along both X chromosomes in hermaphrodites to down-regulate gene expression by half, limiting X-linked gene products to levels produced in XO males. Sequence motifs enriched on the X chromosome play an important role in targeting the DCC to the X. However, these motifs are not strictly X-specific and therefore other factors, such as the chromatin environment of the X chromosome, are likely to aid in DCC targeting. Previously, we found that loss of HTZ-1 results in partial disruption of dosage compensation localization to the X chromosomes. We wanted to know whether other chromatin components coordinated with HTZ-1 to regulate DCC localization. One candidate is DPY-30, a protein known to play a role in DCC localization. DPY-30 homologs in yeast, flies, and mammals are highly conserved members of histone H3 lysine 4 (H3K4) methyltransferase Set1/MLL complexes. Therefore, we investigated the hypothesis that the dosage compensation function of DPY-30 involves H3K4 methylation. We found that in dpy-30 animals the DCC fails to stably bind chromatin. Interestingly, of all the C. elegans homologs of Set1/MLL complex subunits, only DPY-30 is required for stable DCC binding to chromatin. Additionally, loss of H3K4 methylation does not enhance DCC mislocalization in htz-1 animals. We conclude that DPY-30 and HTZ-1 have unique functions in DCC localization, both of which are largely independent of H3K4 methylation. |
| 巻・号 | 6(10) |
| ページ | e25973 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0025973 |
| PII | PONE-D-11-10474 |
| PMID | 21998734 |
| PMC | PMC3187824 |
| MeSH | Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Chromatin / metabolism Dosage Compensation, Genetic / genetics* Histone Methyltransferases Histone-Lysine N-Methyltransferase / deficiency Histones / chemistry* Histones / genetics Histones / metabolism* Lysine* Male Methylation Nuclear Proteins / metabolism* X Chromosome / genetics X Chromosome / metabolism |
| IF | 2.74 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2469 |