Reference - Detail
| RRC ID | 46355 |
|---|---|
| Author | Hoang HD, Prasain JK, Dorand D, Miller MA. |
| Title | A heterogeneous mixture of F-series prostaglandins promotes sperm guidance in the Caenorhabditis elegans reproductive tract. |
| Journal | PLoS Genet |
| Abstract |
The mechanisms that guide motile sperm through the female reproductive tract to oocytes are not well understood. We have shown that Caenorhabditis elegans oocytes synthesize sperm guiding F-series prostaglandins from polyunsaturated fatty acid (PUFA) precursors provided in yolk lipoprotein complexes. Here we use genetics and electrospray ionization tandem mass spectrometry to partially delineate F-series prostaglandin metabolism pathways. We show that omega-6 and omega-3 PUFAs, including arachidonic and eicosapentaenoic acids, are converted into more than 10 structurally related F-series prostaglandins, which function collectively and largely redundantly to promote sperm guidance. Disruption of omega-3 PUFA synthesis triggers compensatory up-regulation of prostaglandins derived from omega-6 PUFAs. C. elegans F-series prostaglandin synthesis involves biochemical mechanisms distinct from those in mammalian cyclooxygenase-dependent pathways, yet PGF(2α) stereoisomers are still synthesized. A comparison of F-series prostaglandins in C. elegans and mouse tissues reveals shared features. Finally, we show that a conserved cytochrome P450 enzyme, whose human homolog is implicated in Bietti's Crystalline Dystrophy, negatively regulates prostaglandin synthesis. These results support the model that multiple cyclooxygenase-independent prostaglandins function together to promote sperm motility important for fertilization. This cyclooxygenase-independent pathway for F-series synthesis may be conserved. |
| Volume | 9(1) |
| Pages | e1003271 |
| Published | 2013-1-1 |
| DOI | 10.1371/journal.pgen.1003271 |
| PII | PGENETICS-D-12-02590 |
| PMID | 23382703 |
| PMC | PMC3561059 |
| MeSH | Animals Arachidonic Acids / chemistry Arachidonic Acids / metabolism Caenorhabditis elegans* / metabolism Caenorhabditis elegans* / physiology Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism Fatty Acids, Omega-3 / chemistry Fatty Acids, Omega-3 / metabolism Fatty Acids, Omega-6 / chemistry Fatty Acids, Omega-6 / metabolism Female Humans Male Mice Oocytes* / metabolism Oocytes* / physiology Prostaglandins F* / biosynthesis Prostaglandins F* / chemistry Reproduction* / genetics Reproduction* / physiology Sperm Motility / genetics Spermatozoa* / metabolism Spermatozoa* / physiology |
| IF | 5.175 |
| Times Cited | 33 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2711 tm3224 |