RRC ID 46358
Author Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, Mizuno K, Tachikawa T, Ishimoto Y, Morishita Y, Tsuji T.
Title Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations.
Journal PLoS One
Abstract The morphologies of ectodermal organs are shaped by appropriate combinations of several deformation modes, such as invagination and anisotropic tissue elongation. However, how multicellular dynamics are coordinated during deformation processes remains to be elucidated. Here, we developed a four-dimensional (4D) analysis system for tracking cell movement and division at a single-cell resolution in developing tooth epithelium. The expression patterns of a Fucci probe clarified the region- and stage-specific cell cycle patterns within the tooth germ, which were in good agreement with the pattern of the volume growth rate estimated from tissue-level deformation analysis. Cellular motility was higher in the regions with higher growth rates, while the mitotic orientation was significantly biased along the direction of tissue elongation in the epithelium. Further, these spatio-temporal patterns of cellular dynamics and tissue-level deformation were highly correlated with that of the activity of cofilin, which is an actin depolymerization factor, suggesting that the coordination of cellular dynamics via actin remodeling plays an important role in tooth epithelial morphogenesis. Our system enhances the understanding of how cellular behaviors are coordinated during ectodermal organogenesis, which cannot be observed from histological analyses.
Volume 11(9)
Pages e0161336
Published 2016-1-1
DOI 10.1371/journal.pone.0161336
PII PONE-D-16-06051
PMID 27588418
PMC PMC5010284
MeSH Animals Cell Division / physiology Cell Movement / physiology Cell Shape / physiology Epithelium / growth & development* Mice Microscopy, Confocal / methods Models, Biological Odontogenesis / physiology* Tooth / growth & development*
IF 2.74
Times Cited 9
Mice RBRC02706 RBRC02707