論文 - 詳細
| RRC ID | 46379 |
|---|---|
| 著者 | Jovanovic M, Reiter L, Clark A, Weiss M, Picotti P, Rehrauer H, Frei A, Neukomm LJ, Kaufman E, Wollscheid B, Simard MJ, Miska EA, Aebersold R, Gerber AP, Hengartner MO. |
| タイトル | RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans. |
| ジャーナル | Genome Res |
| Abstract |
MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression. As miRNAs are involved in a wide range of biological processes and diseases, much effort has been invested in identifying their mRNA targets. Here, we present a novel combinatorial approach, RIP-chip-SRM (RNA-binding protein immunopurification + microarray + targeted protein quantification via selected reaction monitoring), to identify de novo high-confidence miRNA targets in the nematode Caenorhabditis elegans. We used differential RIP-chip analysis of miRNA-induced silencing complexes from wild-type and miRNA mutant animals, followed by quantitative targeted proteomics via selected reaction monitoring to identify and validate mRNA targets of the C. elegans bantam homolog miR-58. Comparison of total mRNA and protein abundance changes in mir-58 mutant and wild-type animals indicated that the direct bantam/miR-58 targets identified here are mainly regulated at the level of protein abundance, not mRNA stability. |
| 巻・号 | 22(7) |
| ページ | 1360-71 |
| 公開日 | 2012-7-1 |
| DOI | 10.1101/gr.133330.111 |
| PII | gr.133330.111 |
| PMID | 22454234 |
| PMC | PMC3396376 |
| MeSH | Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Crosses, Genetic Immunologic Techniques / methods MicroRNAs / genetics MicroRNAs / metabolism* Oligonucleotide Array Sequence Analysis / methods Plasmids / genetics Plasmids / metabolism Protein Biosynthesis Proteomics / methods* RNA Interference RNA Stability RNA, Helminth / genetics RNA, Helminth / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Transgenes |
| IF | 11.093 |
| 引用数 | 15 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm492 |