論文 - 詳細
| RRC ID | 46433 |
|---|---|
| 著者 | Sasagawa Y, Higashitani A, Urano T, Ogura T, Yamanaka K. |
| タイトル | CDC-48/p97 is required for proper meiotic chromosome segregation via controlling AIR-2/Aurora B kinase localization in Caenorhabditis elegans. |
| ジャーナル | J Struct Biol |
| Abstract |
CDC-48/p97 is a AAA (ATPases associated with diverse cellular activities) chaperone involved in protein conformational changes such as the disassembly of protein complexes. We previously reported that Caenorhabditis elegans CDC-48.1 and CDC-48.2 (CDC-48s) are essential for the progression of meiosis I metaphase. Here, we report that CDC-48s are required for proper chromosome segregation during meiosis in C. elegans. In wild-type worms, at the diakinesis phase, phosphorylation of histone H3, one of the known substrates of aurora B kinase (AIR-2), on meiosis I chromatids correlated with AIR-2 localization at the cohesion sites of homologous chromatids. Conversely, depletion of CDC-48s resulted in a significant expansion of signals for AIR-2 and phosphorylated histone H3 over the entire length of meiotic chromosomes, leading to defective chromosome segregation, while the total amount of AIR-2 in lysates was not changed by the depletion of CDC-48s. The defective segregation of meiotic chromosomes caused by the depletion of CDC-48s was suppressed by the simultaneous depletion of AIR-2 and is similar to that observed following the depletion of protein phosphatase 1 (PP1) phosphatases. However, the amount and localization of PP1 were not changed by the depletion of CDC-48s. These results suggest that CDC-48s control the restricted localization of AIR-2 to the cohesion sites of homologous chromatids in meiosis I. |
| 巻・号 | 179(2) |
| ページ | 104-11 |
| 公開日 | 2012-8-1 |
| DOI | 10.1016/j.jsb.2012.06.009 |
| PII | S1047-8477(12)00189-X |
| PMID | 22735043 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Aurora Kinase B Aurora Kinases Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Chromosome Segregation / genetics Chromosome Segregation / physiology* Meiosis / genetics Meiosis / physiology* Protein Serine-Threonine Kinases / metabolism* Valosin Containing Protein |
| IF | 3.071 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm301 tm544 |