Reference - Detail
| RRC ID | 46478 |
|---|---|
| Author | Zanetti S, Grinschgl S, Meola M, Belfiore M, Rey S, Bianchi P, Puoti A. |
| Title | The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA. |
| Journal | RNA |
| Abstract |
Post-transcriptional control regulates many aspects of germline development in the Caenorhabditis elegans hermaphrodite. This nematode switches from spermatogenesis to oogenesis and is, therefore, capable of self-fertilization. This sperm-oocyte switch requires 3' UTR-mediated repression of the fem-3 mRNA. Loss of fem-3 repression results in continuous spermatogenesis in hermaphrodites. Although several factors regulating fem-3 have been identified, little is known about the mechanisms that control fem-3. Here, we investigate the steady-state levels of the fem-3 transcript and the expression pattern of its protein product. We show that FEM-3 is exclusively present in germ cells that are committed to spermatogenesis. We found that in fem-3(gf)/+ heterozygotes, mutant fem-3 gain-of-function transcripts are more abundant than their wild-type counterpart. Furthermore, we show that the penetrance of the fem-3(gf) allele correlates with inefficient FBF binding and extended poly(A) tail size of fem-3 mRNAs. Finally, we show that wild-type and gain-of-function mutated fem-3 mRNAs associate equally well with polyribosomes. We propose that the fem-3 mRNA is regulated through stabilization rather than through translatability. |
| Volume | 18(7) |
| Pages | 1385-94 |
| Published | 2012-7-1 |
| DOI | 10.1261/rna.031237.111 |
| PII | rna.031237.111 |
| PMID | 22635404 |
| PMC | PMC3383969 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Female Gene Expression Gene Expression Regulation, Developmental Genes, Switch* Hermaphroditic Organisms / genetics* Heterozygote Male Oocytes / growth & development* Oogenesis / genetics Oogenesis / physiology Protein Binding RNA, Messenger / metabolism* RNA-Binding Proteins / metabolism* Spermatogenesis / genetics Spermatogenesis / physiology Spermatozoa / growth & development* |
| IF | 4.32 |
| Times Cited | 7 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2435 tm1312 |